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Objective:To investigate the protective effects and possible mechanisms of cortex phellodendri water extract and etha -nol extract on the myocardial injury induced by pituitrin and isoproterenol hydrochloride in rats .Methods:SD rats as the experimental animals were randomly divided into the normal control group , model group , compound Danshen tablets group , phellodendron water ex-tract group and phellodendron ethanol extract group .Pituitrin and isopropyl adrenaline hydrochloride were used to establish the myocar-dial injury model in rats.The serum CK, LDH activity, myocardial tissue SOD activity and MDA content were detected and compared . Results:Compared with those in the normal control group , the serum LDH activity , CK activity and MDA content were significantly in-creased , and the SOD activity in cardiac muscle and myocardial tissue was significantly decreased in the pituitrin -established myocardi-al injury model group (P<0.01).In the isopropyl adrenaline hydrochloride-established myocardial injury model group , the MDA con-tent in myocardial tissue was obviously increased , and the SOD activity in myocardial tissue was decreased obviously (P<0.01).The serum LDH activity, CK activity and MDA content were significantly decreased , and the SOD activity in cardiac muscle and myocardial tissue was increased significantly in all drug-taken groups when compared with those in the pituitrin-established myocardial injury model group, and the differences were statistically significant (P<0.05 or P<0.01).The MDA content in myocardial tissue was significant-ly reduced , and the SOD activity was increased significantly in all drug-taken groups when compared with those in the isopropyl adrena-line hydrochloride-established myocardial injury model group , and the differences were statistically significant (P<0.05 or P<0.01). Conclusion:Cortex phellodendri extract has certain protective effect on myocardial injury induced by pituitrin and isopropyl adrenaline hydrochloride in rats .
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Objective To investigate the effects of down-regulation of Beclin 1, which is an autophagy regulatory molecule, expression induced by RNA interference on the proliferation and apoptosis in skin fibroblasts of naked mole rat. Methods The expression levels of Beclin 1 were detected after starvation or H2 O2 treatment.The fibroblasts were transi-ently transfected with specific siRNA targeting Beclin 1 and then screened by real-time PCR and Western blot.Cell prolifer-ation and apoptosis were determined using CCK-8 detection kit and flow cytometry ( FCM ) .The expressions of related genes were detected by Western blot.Results The expression of Beclin 1 gene at mRNA and protein levels was signifi-cantly lower in fibroblasts of the naked mole rat.Starvation and H2 O2 treatment induced changes of the Beclin 1 expression. Inhibition of Beclin 1 gene expression can inhibit cell proliferation and induce early and late apoptosis.The protein levels of p53, BAX, Bcl2, LC3B, p-AKT and mTOR were reduced after transient transfection with Beclin 1-siRNA.Conclusions The expression of Beclin 1 in fibroblasts of naked mole rat are changed in response to starvation or H2 O2 stimulation.Inhi-bition of Beclin 1 gene expression can inhibit cell proliferation and induce apoptosis.Therefore, Beclin1 gene may play a regulatory role in autophagy, proliferation and apoptosis in the skin fibroblasts of naked mole rat.
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Objective:To study the anti-inflammatory and analgesic effects of different solvent extracts of Folium Pyrrosiae. Meth-ods:Water extract and 75% alcohol extract of Folium Pyrrosiae were obtained. Mouse auricle swelling model induced by xylene was used to observe the anti-inflammation. The analgesic effect was tested by acetic acid writhing test and hot plate test. Results:The eth-anol extract of Folium Pyrrosiae could markedly inhibit the mouse auricle swelling induced by xylene (P<0. 01), and had the ability to inhibit the twisting induced by acetic acid in the mice (P <0. 05). The ethanol extract of Folium Pyrrosiae could increase the threshold of pain in the mice significantly after the 1-hour and 2-hour treatment (P<0. 05). The water extract of Folium Pyrrosiae could inhibit the mouse auricle swelling induced by xylene and the writhing reaction induced by acetic acid (P<0. 05). The water ex-tract of Folium Pyrrosiae could increase the threshold of pain in the mice significantly after the 1-hour treatment (P<0. 05). Conclu-sion:Folium Pyrrosiae has obvious analgesic and anti-inflammatory effects.
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Methods and significances of the laboratory opening management for the function experiment teaching center are introduced in this paper. The problems existing in the management of the open Lab and the thinking about the management are communicated with the peers to provide the reference for the opened lab.
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Objective To explore the environmental factors change on the plasma endothelin(ET) and nitric oxide(NO) in rabbits exposed to a complex environment:high temperature,hunger,and so on.Methods Ninety China rabbits were randomly divided into myocardial ischemia group,myocardial ischemia and hypoxia and high temperature group,myocardial ischemia and hypoxia group,myocardial ischemia and hunger group,myocardial ischemia and hypoxia and high temperature and hunger group,hunger group,hypoxia group,high temperatures group,control group,ten rabbits in each group.The animal myocardial ischemia was caused by fulgurating the left anterior descending branch artery in the heart.The animal hypoxia was caused by breathing in 8.5% nitrogen oxygen atmosphere(nitrogen content was 91.5%,oxygen content was 8.5%).The animals in the high temperature group were put into a high temperature [(42?2)℃] environment.The animals in the hunger group were treated with fasting and water deprivation for 24 h.Every animal has been taken in above-mentioned experiment environment for 30 min.Blood from jugular veins collected,and the contents of plasma ET and NO were determine.Results Compared with control group,except the hunger group and high temperature group,ET levels in the others six group were significantly increased(P
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In order to make good use of laboratory opening,the way,method together with the effect of laboratory opening in our college were analyzed and discussed in this paper. The perfect practice effect shows that laboratory opening plays an important role in cultivation for independent research abilities and scientific innovative abilities of undergraduates.
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To discuss the course of implementing tutorial system as well as the effect on medical students,and to point out that implementing teacher system is a kind of pattern of training innovation type of medical talents.
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We provide laboratory for students to design and complete experiment independently,and examine their operation ability in course of experiment.After accomplishing their experiments,the students must write a disquisition on it.Through this exploration experimentation,we have trained students scientific analysis ability and innovation ability and make them benefit form it in their future study and work.
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BACKGROUND: Previously, there are some researches on studying the effects of hypoxia or hypothermia singly. Nowadays, study on the synthetic action of hypoxia and hypothermia has been attached widely importance.OBJECTIVE: To explore the combination effects of acute hypothermia and hypoxia on superoxide dismutase(SOD) and malondialdehyde in normal rabbits and rabbits with myocardial ischemia.DESIGN: Randomized controlled trial.SETTING, MATERIALS and INTERVENTIONS: Experimental site:Physiological Laboratory of Youjiang Ethnic Medical College. A total of 32 healthy rabbits weighed between 1.8to 2.5 kg were chosen. They were randomly divided into 4 groups each with 8 rabbits: normal group, hypothermia and hypoxia group, ischemic group with normal temperature and oxygen,ischemic group with hypothermia and hypoxia. Normal rabbits and ischemic rabbits were put in fridge at low temperature( -10 ± 2) ℃ and low oxygen (oxygen content of 8.5% ) for one hour and tested activity of SOD and concentration of malondialdehyde in serum.MAIN OUTCOME MEASURES: Comparison of serum SOD and malondialdehyde between normal rabbits and myocardial ischemic rabbits under the acute hypothermia and hypoxia conditions.RESULTS: The SOD level of rabbits in hypothermia and hypoxia group [(371.04 ± 29.96) kNU/L] was much lower than that of normal temperature and oxygen group[ (424.09 ± 22.59) kNU/L] ( t = 5.21, P < 0.01) while the content of malondialdehyde[ (5.58 ± 0. 44) μ mol/L] was greater than that of normal group[ (4.44 ± 1.11) μ mol/L] ( t = 3.21, P < 0.05) . In myocardial ischemia group with hypothermia and hypoxia, SOD was greatly lower than that of myocardial ischemia group with normal temperature and oxygen(t=4.37, P < 0.01) while the malondialdehyde was greatly higher than that of ischemic group with normal temperature and oxygen group(t=2.94, P <0.05).CONCLUSION: The combined effects of acute hypothermia and hypoxia will aggravate the damage to body.
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Objective To observe the effect of environmental factors change on the hemorheology of the rabbit with myocardial ischemia.Methods The rabbits were divided into six groups,the group of myocardial ischemia(myocardial ischemia operation),the group of myocardial ischemia with hypoxia(volume fraction of oxygen of 91.5% nitrogen and 8.5% oxygen) and high temperature [(42?2)℃],the group of myocardial ischemia with hypoxia(volume of fraction of oxygen of 91.5% nitrogen and 8.5% oxygen),the group of myocardial ischemia with hypoxia(volume of fraction of oxygen of 91.5% nitrogen and 8.5% oxygen) and high temperature[(42?2)℃] and hunger(forbid water and fast in 24 h),the group of myocardial ischemia with hunger,control group,10 in each.After 30 minutes of treatment,the indexes of the hemorheology were determined.Results The changed environmental factors,hypoxia,high temperature,hunger,presented an increasing effect on the blood viscosity,plasma viscosity,blood high shear reduced viscosity,blood low shear reduced viscosity and equation K value of ESR(P
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Objective To explore the effects of alcohol on NO concentration in plasma and activity of ATP enzyme in erythrocytes of rabbits. Methods 32 rabbits were divided into groups and treated with alcohol, injected intravenously, at the doses of 0.5 g/kg, 1.0 g/kg and 1.5 g/kg, 15 minutes after treatment, the blood samples were collected and NO concentration in plasma, activity of ATP enzyme in erythrocytes were determined. Results NO concentration in plasma of rabbits significantly decreased in 0.5 g/kg and 1.0 g/kg groups, but increased in 1.5 g/kg group (P
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Objective To study the effects of acute low temperature and hypoxia on serum SOD and MDA of myocardial ischemia rabbit. Methods 24 rabbits were randomly divided into 3 groups, such as normally low tamperature and hypoxia group, myocardial ischemia normal temporature and normal oxygen group and myocardial ischemia low temporature and hypoxia group. Put myocardial ischemia rabbits into the condition in which low temperature(-10?2) ℃ and hypoxia (oxygen content 8.5% ) existed, superoxide dismutase (SOD) activity and malondialdehyde (MDA) contents were measured after an hour. Results Serum SOD activity were decreased significantly in acute low temperature and hypoxia compared with normal temperature and normal oxygen pressure (P